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In Vivo Analysis of Calcium Levels and Glutathione Redox Status in Arabidopsis Epidermal Leaf Cells Infected with the Hypersensitive Response-Inducing Bacteria Pseudomonas syringae pv. tomato AvrB (PstAvrB).
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1743, pp. 125-141. - Publication Year :
- 2018
-
Abstract
- Plants react to the attack of pathogen microorganisms by mounting appropriate and efficient downstream defense responses often involving a form of localized cell death called hypersensitive response (HR).Here we describe an innovative and noninvasive protocol based on in vivo bioimaging technique coupled with utilization of genetically encoded fluorescent sensors that allows to monitor and analyze intracellular calcium (Ca <superscript>2+</superscript> ) dynamics and changes of the glutathione redox status taking place in plant organs during plant interaction with the HR-inducing bacteria Pseudomonas syringae (PstAvrB).
- Subjects :
- Gene Expression
Genes, Reporter
Microscopy, Fluorescence
Molecular Imaging
Phenotype
Reactive Oxygen Species metabolism
Reproducibility of Results
Arabidopsis metabolism
Arabidopsis microbiology
Calcium metabolism
Glutathione metabolism
Oxidation-Reduction
Plant Leaves metabolism
Plant Leaves microbiology
Pseudomonas syringae physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1743
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 29332292
- Full Text :
- https://doi.org/10.1007/978-1-4939-7668-3_12