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Direct interaction between VRN1 protein and the promoter region of the wheat FT gene.
- Source :
-
Genes & genetic systems [Genes Genet Syst] 2018 Jul 13; Vol. 93 (1), pp. 25-29. Date of Electronic Publication: 2018 Jan 17. - Publication Year :
- 2018
-
Abstract
- The wheat florigen gene Wheat FLOWERING LOCUS T (WFT, which is identical to VRN3) is an integrator of the vernalization, photoperiod and autonomous pathways in wheat flowering. Many studies have indicated that VERNALIZATION 1 (VRN1) directly or indirectly up-regulates WFT expression in leaves. VRN1 encodes an APETALA1/FRUITFULL-like MADS box transcription factor that is up-regulated by vernalization and aging, leading to promotion of flowering. In this study, the VRN1 protein was expressed as a His-Tag fusion protein in Escherichia coli and used in an electrophoretic mobility shift assay (EMSA). The results from the EMSA indicated that the VRN1 protein directly binds to the CArG-box in the promoter region of WFT, suggesting the direct up-regulation of WFT by VRN1 in the leaves of wheat plants.
Details
- Language :
- English
- ISSN :
- 1880-5779
- Volume :
- 93
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genes & genetic systems
- Publication Type :
- Academic Journal
- Accession number :
- 29343669
- Full Text :
- https://doi.org/10.1266/ggs.17-00041