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nextPARS: parallel probing of RNA structures in Illumina.

nextPARS: parallel probing of RNA structures in Illumina.

Authors :
Saus E
Willis JR
Pryszcz LP
Hafez A
Llorens C
Himmelbauer H
Gabaldón T
Source :
RNA (New York, N.Y.) [RNA] 2018 Apr; Vol. 24 (4), pp. 609-619. Date of Electronic Publication: 2018 Jan 22.
Publication Year :
2018

Abstract

RNA molecules play important roles in virtually every cellular process. These functions are often mediated through the adoption of specific structures that enable RNAs to interact with other molecules. Thus, determining the secondary structures of RNAs is central to understanding their function and evolution. In recent years several sequencing-based approaches have been developed that allow probing structural features of thousands of RNA molecules present in a sample. Here, we describe nextPARS, a novel Illumina-based implementation of in vitro parallel probing of RNA structures. Our approach achieves comparable accuracy to previous implementations, while enabling higher throughput and sample multiplexing.<br /> (© 2018 Saus et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.)

Details

Language :
English
ISSN :
1469-9001
Volume :
24
Issue :
4
Database :
MEDLINE
Journal :
RNA (New York, N.Y.)
Publication Type :
Academic Journal
Accession number :
29358234
Full Text :
https://doi.org/10.1261/rna.063073.117