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ATP-mediated Events in Peritubular Cells Contribute to Sterile Testicular Inflammation.
- Source :
-
Scientific reports [Sci Rep] 2018 Jan 23; Vol. 8 (1), pp. 1431. Date of Electronic Publication: 2018 Jan 23. - Publication Year :
- 2018
-
Abstract
- Peritubular myoid cells, which form the walls of seminiferous tubules in the testis, are functionally unexplored. While they transport sperm and contribute to the spermatogonial stem cell niche, specifically their emerging role in the immune surveillance of the testis and in male infertility remains to be studied. Recently, cytokine production and activation of Toll-like receptors (TLRs) were uncovered in cultured peritubular cells. We now show that human peritubular cells express purinergic receptors P2RX4 and P2RX7, which are functionally linked to TLRs, with P2RX4 being the prevalent ATP-gated ion channel. Subsequent ATP treatment of cultured peritubular cells resulted in up-regulated (pro-)inflammatory cytokine expression and secretion, while characteristic peritubular proteins, that is smooth muscle cell markers and extracellular matrix molecules, decreased. These findings indicate that extracellular ATP may act as danger molecule on peritubular cells, able to promote inflammatory responses in the testicular environment.
- Subjects :
- Adult
Biomarkers metabolism
Cells, Cultured
Cytokines genetics
Extracellular Matrix Proteins genetics
Extracellular Matrix Proteins metabolism
Humans
Infertility, Male immunology
Male
Middle Aged
Receptors, Purinergic P2X4 metabolism
Receptors, Purinergic P2X7 metabolism
Seminiferous Tubules immunology
Adenosine Triphosphate pharmacology
Cytokines metabolism
Gene Regulatory Networks
Infertility, Male metabolism
Seminiferous Tubules metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29362497
- Full Text :
- https://doi.org/10.1038/s41598-018-19624-3