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Multilocus Sequence Typing Reveals both Shared and Unique Genotypes of Cryptococcus neoformans in Jiangxi Province, China.
- Source :
-
Scientific reports [Sci Rep] 2018 Jan 24; Vol. 8 (1), pp. 1495. Date of Electronic Publication: 2018 Jan 24. - Publication Year :
- 2018
-
Abstract
- Cryptococcosis is a globally distributed infectious fungal disease. However, much remains unknown about its molecular epidemiology in many parts of the world. In this study, we analyzed 86 clinical Cryptococcus neoformans isolates from 14 regions in Jiangxi Province in south central China. Each isolate was from a different patient and 35 of the 86 (40.7%) patients were infected with HIV. All strains belonged to serotype A and mating type α (MATα). Genotyping based on DNA sequences at seven nuclear loci revealed eight sequence types (STs) among the 86 isolates, including two novel STs that have not been reported from other parts of the world. ST5 was the dominant genotype and our comparative analyses showed that these genotypes in Jiangxi likely originated by dispersal from other regions within and outside of China and/or mutations from another genotype within Jiangxi. Though none of the isolates was resistant to the five tested antifungal drugs (flucytosine, amphotericin B, fluconazole, itraconazole, and voriconazole), obvious differences in their minimum inhibitory concentrations were observed, even among isolates of the same ST. Our results suggest that continuous monitoring should be conducted to understand the changing dynamics of C. neoformans in this and other regions.
- Subjects :
- Adolescent
Adult
Aged
Child
Child, Preschool
China epidemiology
Cryptococcosis genetics
Cryptococcosis microbiology
Cryptococcus neoformans classification
Cryptococcus neoformans isolation & purification
Female
Genotype
Humans
Male
Middle Aged
Multilocus Sequence Typing
Phylogeny
Serotyping
Young Adult
Antifungal Agents pharmacology
Cryptococcosis epidemiology
Cryptococcus neoformans genetics
Drug Resistance, Fungal genetics
Genes, Mating Type, Fungal
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29367679
- Full Text :
- https://doi.org/10.1038/s41598-018-20054-4