Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe.

Authors :: Ali F
Aute S
Sreedharan S
Anila HA
Saeed HK
Smythe CG
Thomas JA
Das A
Source :: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2018 Feb 15; Vol. 54 (15), pp. 1849-1852.
Publication Year :: 2018
Abstract: BODIPY derivative, SF-1, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles. SF-1 is compatible with 3D-SIM imaging of individual cells.

Subjects :: Animals
Imaging, Three-Dimensional
Mice
RAW 264.7 Cells
Time Factors
Boron Compounds chemistry
Fluorescent Dyes chemistry
Hypochlorous Acid analysis
Macrophages chemistry
Microscopy methods
Organelles chemistry

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