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A 60 kDa prolactin variant secreted by cervical cancer cells modulates apoptosis and cytokine production.
- Source :
-
Oncology reports [Oncol Rep] 2018 Mar; Vol. 39 (3), pp. 1253-1260. Date of Electronic Publication: 2018 Jan 19. - Publication Year :
- 2018
-
Abstract
- Prolactin (PRL) is associated with different types of cancer, such as cervical cancer. Recombinant PRL has antiapoptotic effect on cervical cancer cells, and it can also induce cytokine production on macrophages. A 60 kDa variant of PRL is produced by cervical cancer cells. The aim of the present study was to evaluate this variant's bioactivity, to test its effect on cervical cancer cell apoptosis, and to assess its ability to induce cytokine production on THP-1 macrophages. First, 60 kDa PRL was isolated and used to stimulate Nb2 cells. Later, apoptosis was measured after exposure to 60 kDa PRL. Finally, cytokines were measured on THP-1 stimulated supernatants. Our results show that 60 kDa PRL increased Nb2 cell proliferation. Apoptosis was decreased after stimuli with 60 kDa PRL in cervical cancer cells. IL-1β and TNF-α are produced by THP-1 macrophages after stimuli. These results suggest that 60 kDa PRL produced by cervical cancer cells is able to reduce apoptosis in HeLa, SiHa and C-33A cells and induce IL-1β and TNF-α production by THP-1 macrophages.
- Subjects :
- Animals
Cell Line
Cell Line, Tumor
Female
HeLa Cells
Humans
Interleukin-1beta biosynthesis
Macrophages immunology
Prolactin isolation & purification
Prolactin metabolism
Protein Isoforms isolation & purification
Protein Isoforms metabolism
Protein Isoforms physiology
Rats
Tumor Necrosis Factor-alpha biosynthesis
Apoptosis
Cytokines biosynthesis
Prolactin physiology
Uterine Cervical Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2431
- Volume :
- 39
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 29399697
- Full Text :
- https://doi.org/10.3892/or.2018.6222