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Resistance to Bacillus thuringiensis linked with a cadherin transmembrane mutation affecting cellular trafficking in pink bollworm from China.
- Source :
-
Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 2018 Mar; Vol. 94, pp. 28-35. Date of Electronic Publication: 2018 Feb 02. - Publication Year :
- 2018
-
Abstract
- Evolution of pest resistance reduces the efficacy of insecticidal proteins from the gram-positive bacterium Bacillus thuringiensis (Bt) used widely in sprays and transgenic crops. In some previously studied strains of three major lepidopteran pests, resistance to Bt toxin Cry1Ac is associated with mutations disrupting the extracellular or cytoplasmic domains of cadherin proteins that bind Cry1Ac in the midgut of susceptible larvae. Here we report the first case of a cadherin transmembrane mutation associated with insect resistance to Bt. We discovered this mutation in a strain of the devastating global cotton pest, the pink bollworm (Pectinophora gossypiella), derived from a field population in the Yangtze River Valley of China. The mutant allele analyzed here has a 207 base pair deletion and encodes a cadherin protein lacking its transmembrane domain. Relative to a susceptible strain, a strain homozygous for this allele had 220-fold resistance to Cry1Ac and 2.1-fold cross-resistance to Cry2Ab. On transgenic cotton plants producing Cry1Ac, no susceptible larvae survived, but the resistant strain completed its life cycle. Inheritance of resistance to Cry1Ac was autosomal, recessive and tightly linked with the cadherin gene. Transportation of cadherin protein to the cell membrane and susceptibility to Cry1Ac occurred in transfected insect cells expressing the wild type cadherin allele, but not in transfected insect cells expressing the mutant cadherin allele. The results imply that the mutant allele analyzed here confers resistance to Cry1Ac by disrupting cellular trafficking of cadherin.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Cadherins metabolism
China
Female
Gossypium genetics
Gossypium growth & development
Insect Proteins metabolism
Larva drug effects
Larva genetics
Larva growth & development
Larva physiology
Male
Moths growth & development
Moths physiology
Mutation
Plants, Genetically Modified genetics
Plants, Genetically Modified growth & development
Protein Transport
Bacillus thuringiensis chemistry
Cadherins genetics
Insect Proteins genetics
Insecticide Resistance genetics
Insecticides adverse effects
Moths drug effects
Moths genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0240
- Volume :
- 94
- Database :
- MEDLINE
- Journal :
- Insect biochemistry and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 29408651
- Full Text :
- https://doi.org/10.1016/j.ibmb.2018.01.004