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Propionibacterium acnes-Derived Extracellular Vesicles Promote Acne-Like Phenotypes in Human Epidermis.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 2018 Jun; Vol. 138 (6), pp. 1371-1379. Date of Electronic Publication: 2018 Jan 31. - Publication Year :
- 2018
-
Abstract
- Acne vulgaris is an inflammatory disease occurring in the pilosebaceous unit and is the most common skin condition in young people. A gram-positive bacterium, Propionibacterium acnes, has been suspected to contribute to the development of acne. Here, we report that P. acnes constitutively releases extracellular vesicles (EVs) exhibiting typical EV morphology and size. Moreover, the P. acnes-derived EVs (PEVs) can induce acne-like phenotypes in human epidermal keratinocytes and a reconstituted human skin model. PEVs significantly induced inflammatory cytokines IL-8 and GM-CSF and dysregulated epidermal differentiation by increasing proliferating keratinocytes and decreasing epidermal keratin 10 and desmocollin 1 levels. PEVs showed strong effects, evoking these responses at earlier time points compared with P. acnes extract at the same protein concentration. We verified that PEVs were internalized via clathrin-dependent endocytosis into keratinocytes and that PEV-induced cellular responses occurred via Toll-like receptor 2-dependent signal cascades. Furthermore, PEVs showed a stronger effect than keratinocytes in inducing inflammatory cytokines in myeloid cells. Collectively, our study suggests that PEVs induce acne-like phenotypes in a unique way; therefore, inhibiting the release of EVs from P. acnes or targeting PEV-mediated signaling pathways could represent an alternative method for alleviating acne occurrence and phenotypes.<br /> (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Acne Vulgaris microbiology
Acne Vulgaris pathology
Cell Line
Epidermal Cells
Epidermis immunology
Epidermis metabolism
Extracellular Vesicles metabolism
Gram-Positive Bacterial Infections microbiology
Gram-Positive Bacterial Infections pathology
Humans
Keratinocytes immunology
Keratinocytes metabolism
Myeloid Cells immunology
Primary Cell Culture
Propionibacterium acnes cytology
Propionibacterium acnes metabolism
RNA, Small Interfering metabolism
Signal Transduction immunology
Toll-Like Receptor 2 genetics
Toll-Like Receptor 2 metabolism
Acne Vulgaris immunology
Extracellular Vesicles immunology
Gram-Positive Bacterial Infections immunology
Propionibacterium acnes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1523-1747
- Volume :
- 138
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 29409885
- Full Text :
- https://doi.org/10.1016/j.jid.2018.01.007