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Phytosynthesized silver nanoparticles as antiquorum sensing and antibiofilm agent against the nosocomial pathogen Serratia marcescens: an in vitro study.
- Source :
-
Journal of applied microbiology [J Appl Microbiol] 2018 Jun; Vol. 124 (6), pp. 1425-1440. Date of Electronic Publication: 2018 Mar 23. - Publication Year :
- 2018
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Abstract
- Aim: Serratia marcescens is an important multidrug-resistant human pathogen. The pathogenicity of S. marcescens mainly depends on the quorum sensing (QS) mechanism, which regulates the virulence factors production and biofilm formation. Hence, targeting QS mechanism in S. marcescens will ultimately pave the way to combat its pathogenicity. Thus, the present study is intended to evaluate the efficacy of Vetiveria zizanioides root extract-mediated silver nanoparticles (AgNPs) as a potent anti-QS and antibiofilm agent against S. marcescens.<br />Methods and Results: The AgNPs were synthesized using V. zizanioides aqueous root extract and the physiochemical properties of V. zizanioides-based AgNPs (VzAgNPs) were evaluated using analytical techniques such as ultraviolet-visible absorption spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, dynamic light scattering and scanning and transmission electron microscopic techniques. VzAgNPs were found to attenuate the QS-dependent virulence factors, namely prodigiosin, protease, lipase, exopolysaccharide productions and biofilm formation of S. marcescens, without inhibiting its growth. Further, the transcriptomic analysis confirmed the down-regulation of QS-dependent genes, which encode for the production of virulence factors and biofilm formation.<br />Conclusion: The current study confirms VzAgNPs as an ideal anti-QS and antibiofilm agent against S. marcescens.<br />Significance and Impact of the Study: This is the first approach that validates the anti-QS and antibiofilm potential of phytosynthesized VzAgNPs against the nosocomial pathogen, S. marcescens. As VzAgNPs exhibits potent antivirulent activities, it could be used to treat hospital-acquired S. marcescens infections.<br /> (© 2018 The Society for Applied Microbiology.)
- Subjects :
- Anti-Bacterial Agents chemistry
Anti-Bacterial Agents pharmacology
Biofilms growth & development
Chrysopogon metabolism
Humans
Quorum Sensing drug effects
Serratia marcescens genetics
Serratia marcescens physiology
Silver chemistry
Silver metabolism
Virulence Factors genetics
Virulence Factors metabolism
Anti-Bacterial Agents metabolism
Biofilms drug effects
Chrysopogon chemistry
Cross Infection microbiology
Metal Nanoparticles chemistry
Serratia marcescens drug effects
Silver pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2672
- Volume :
- 124
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 29431875
- Full Text :
- https://doi.org/10.1111/jam.13728