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A Graphene Quantum Dots-Enzyme Hybrid System for the Fluorescence Assay of Alkaline Phosphatase Activity and Inhibitor Screening.
- Source :
-
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2018; Vol. 34 (2), pp. 131-136. - Publication Year :
- 2018
-
Abstract
- A graphene quantum dots (GQDs) and horse radish peroxidase (HRP) hybrid system was designed for the sensing of alkaline phosphatase (ALP) activity and inhibitor screening. We found that the photoluminescence (PL) intensity of GQDs could be quenched efficiently in the presence of phenol, H <subscript>2</subscript> O <subscript>2</subscript> and HRP. Moreover, ALP could hydrolyze disodium phenyl phosphate (DPP) to produce phenol, and also could result in the photoluminescence quenching of GQDs. The decrease in the PL intensity was linear to the activity of ALP in the concentration range of 0.02 - 0.8 U/L, with a detection limit of 0.008 U/L. The proposed GQDs/HRP hybrid system was successfully applied to ALP determination in human serum samples. The inhibition study was further analyzed, and Na <subscript>3</subscript> VO <subscript>4</subscript> (as an ALP inhibitor) showed a clear inhibition effect. The results suggest that the GQDs/HRP hybrid system has good potential applications for the assay of ALP activity and inhibitors screening in related biochemical fields.
- Subjects :
- Enzyme Inhibitors blood
Horseradish Peroxidase metabolism
Humans
Hydrogen-Ion Concentration
Phenol chemistry
Spectrometry, Fluorescence
Time Factors
Alkaline Phosphatase antagonists & inhibitors
Alkaline Phosphatase metabolism
Drug Evaluation, Preclinical methods
Enzyme Assays methods
Enzyme Inhibitors pharmacology
Graphite chemistry
Quantum Dots chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1348-2246
- Volume :
- 34
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 29434096
- Full Text :
- https://doi.org/10.2116/analsci.34.131