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First evidence for a multienzyme complex of lipid biosynthesis pathway enzymes in Cunninghamella bainieri.
- Source :
-
Scientific reports [Sci Rep] 2018 Feb 15; Vol. 8 (1), pp. 3077. Date of Electronic Publication: 2018 Feb 15. - Publication Year :
- 2018
-
Abstract
- Malic enzyme (ME) plays a vital role in determining the extent of lipid accumulation in oleaginous fungi being the major provider of NADPH for the activity of fatty acid synthase (FAS). We report here the first direct evidence of the existence of a lipogenic multienzyme complex (the lipid metabolon) involving ME, FAS, ATP: citrate lyase (ACL), acetyl-CoA carboxylase (ACC), pyruvate carboxylase (PC) and malate dehydrogenase (MDH) in Cunninghamella bainieri 2A1. Cell-free extracts prepared from cells taken in both growth and lipid accumulation phases were prepared by protoplasting and subjected to Blue Native (BN)-PAGE coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). A high molecular mass complex (approx. 3.2 MDa) consisting of the above enzymes was detected during lipid accumulation phase indicating positive evidence of multienzyme complex formation. The complex was not detected in cells during the balanced phase of growth or when lipid accumulation ceased, suggesting that it was transiently formed only during lipogenesis.
- Subjects :
- ATP Citrate (pro-S)-Lyase metabolism
Acetyl-CoA Carboxylase metabolism
Chromatography, Liquid methods
Fatty Acid Synthase, Type II metabolism
Fatty Acid Synthases metabolism
Fatty Acids metabolism
Lipid Metabolism physiology
Lipogenesis physiology
Malate Dehydrogenase metabolism
Malates metabolism
Pyruvate Carboxylase metabolism
Tandem Mass Spectrometry methods
Cunninghamella enzymology
Cunninghamella metabolism
Lipids biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29449592
- Full Text :
- https://doi.org/10.1038/s41598-018-21452-4