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Absence of relationship between type-I interferon suppression and neuropathogenicity of EHV-1.
- Source :
-
Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2018 Mar; Vol. 197, pp. 24-30. - Publication Year :
- 2018
-
Abstract
- Equine herpesvirus-1 (EHV-1) infection is an important and highly prevalent disease in equine populations worldwide. Previously we have demonstrated that a neuropathogenic strain of EHV-1, T953, suppresses the host cell's antiviral type-I interferon (IFN) response in vitro. Whether or not this is unique to EHV-1 strains possessing the neuropathogenic genotype has been undetermined. Here, we examined whether there is any direct relationship between neuropathogenic genotype and the induced IFN-β response in equine endothelial cells (EECs) infected with 10 different strains of EHV-1. The extent of virus cell-to-cell spread following infection in EECs was also compared between the neuropathogenic and the non-neuropathogenic genotype of EHV-1. We then compared IFN-β and the total type-I IFN protein suppression between T953, an EHV-1 strain that is neuropathogenic and T445, an EHV-4 strain mainly associated only with respiratory disease. Data from our study revealed no relationship between the neuropathogenic genotype of EHV-1 and the induced IFN-β mRNA by the host cell. Results also indicate no statistically significant difference in plaque sizes of both genotypes of EHV-1 produced in EECs. However, while the T953 strain of EHV-1 was able to suppress IFN-β mRNA and type-I IFN biological activity at 12 h post-infection (hpi), EHV-4 weakly induces both IFN-β mRNA and type-I IFN biological activity. This finding correlated with a statistically significant difference in the mean plaque sizes produced by the two EHV subtypes in EECs. Our data help illuminate how EHV-1, irrespective of its genotype, evades the host cell's innate immune response thereby enabling viral spread to susceptible cells.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Endothelial Cells immunology
Endothelial Cells virology
Genotype
Herpesviridae Infections immunology
Herpesvirus 1, Equid classification
Horse Diseases virology
Horses immunology
Immune Evasion
Immunity, Innate
Interferon Type I immunology
Herpesviridae Infections veterinary
Herpesvirus 1, Equid pathogenicity
Horse Diseases immunology
Interferon-beta immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2534
- Volume :
- 197
- Database :
- MEDLINE
- Journal :
- Veterinary immunology and immunopathology
- Publication Type :
- Academic Journal
- Accession number :
- 29475503
- Full Text :
- https://doi.org/10.1016/j.vetimm.2018.01.007