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An all-trans-retinal-binding opsin peropsin as a potential dark-active and light-inactivated G protein-coupled receptor.
- Source :
-
Scientific reports [Sci Rep] 2018 Feb 23; Vol. 8 (1), pp. 3535. Date of Electronic Publication: 2018 Feb 23. - Publication Year :
- 2018
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Abstract
- Peropsin or retinal pigment epithelium-derived rhodopsin homolog, found in many animals, belongs to the opsin family. Most opsins bind to 11-cis-retinal as a chromophore and act as light-activated G protein-coupled receptors. Some peropsins, however, bind all-trans-retinal and isomerise it into 11-cis form by light, and peropsin has been suggested to supply other visual opsins with 11-cis-retinal. Additionally, peropsin has some amino acid sequence motifs that are highly conserved among G protein-coupled opsins. Here, using chimeric mutant peropsins, we found that peropsin potentially generates an "active form" that drives G-protein signalling in the dark by binding to all-trans-retinal and that the active form photo-converts to an inactive form containing 11-cis-retinal. Comparative spectroscopic analysis demonstrated that spider peropsin exhibited catalytic efficiency for retinal photoisomerisation that was much lower than a retinal photoisomerase, squid retinochrome. The chimeric peropsins, constructed by replacing the third intracellular loop region with that of Gs- or Gi-coupled opsin, were active and drove Gs- or Gi-mediated signalling in the dark, respectively, and were inactivated upon illumination in mammalian cultured cells. These results suggest that peropsin acts as a dark-active, light-inactivated G protein-coupled receptor and is useful as a novel optogenetic tool.
- Subjects :
- Amino Acid Motifs
Animals
Calcium metabolism
Cations, Divalent
Cyclic AMP metabolism
Decapodiformes
Gene Expression
HEK293 Cells
Humans
Lancelets
Light Signal Transduction
Mutant Chimeric Proteins metabolism
Optogenetics methods
Photoperiod
Plasmids chemistry
Plasmids metabolism
Retinal Pigments metabolism
Retinaldehyde chemistry
Rhodopsin metabolism
Spiders
Stereoisomerism
Transfection
Mutant Chimeric Proteins genetics
Retinal Pigments genetics
Retinaldehyde metabolism
Rhodopsin genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29476064
- Full Text :
- https://doi.org/10.1038/s41598-018-21946-1