Back to Search
Start Over
Complete genomic analysis of multidrug-resistance Pseudomonas aeruginosa Guangzhou-Pae617, the host of megaplasmid pBM413.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2018 Apr; Vol. 117, pp. 265-269. Date of Electronic Publication: 2018 Feb 24. - Publication Year :
- 2018
-
Abstract
- Objectives: We previously described the novel qnrVC6 and bla <subscript>IMP-45</subscript> carrying megaplasmid pBM413. This study aimed to investigate the complete genome of multidrug-resistance P. aeruginosa Guangzhou-Pae617, a clinical isolate from the sputum of a patient who was suffering from respiratory disease in Guangzhou, China.<br />Methods: The genome was sequenced using Illumina Hiseq 2500 and PacBio RS II sequencers and assembled de novo using HGAP. The genome was automatically and manually annotated.<br />Results: The genome of P. aeruginosa Guangzhou-Pae617 is 6,430,493 bp containing 5881 predicted genes with an average G + C content of 66.43%. The genome showed high similarity to two new sequenced P. aeruginosa strains isolated from New York, USA. From the whole genome sequence, we identified a type IV pilin, two large prophages, 15 antibiotic resistant genes, 5 genes involved in the "Infectious diseases" pathways, and 335 virulence factors.<br />Conclusions: The antibiotic resistance and virulence factors in the genome of P. aeruginosa strain Guangzhou-Pae617 were identified by complete genomic analysis. It contributes to further study on antibiotic resistance mechanism and clinical control of P. aeruginosa.<br /> (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Subjects :
- Base Composition
China
DNA, Bacterial genetics
Fimbriae Proteins genetics
Humans
Peptide Fragments genetics
Phylogeny
Plasmids genetics
Sequence Analysis, DNA
Sputum microbiology
Virulence Factors genetics
Drug Resistance, Multiple, Bacterial genetics
Genes, Bacterial genetics
Genome, Bacterial
Pseudomonas Infections microbiology
Pseudomonas aeruginosa genetics
Pseudomonas aeruginosa isolation & purification
Whole Genome Sequencing
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 117
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 29486277
- Full Text :
- https://doi.org/10.1016/j.micpath.2018.02.049