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Arabidopsis RETICULON-LIKE3 (RTNLB3) and RTNLB8 Participate in Agrobacterium-Mediated Plant Transformation.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2018 Feb 24; Vol. 19 (2). Date of Electronic Publication: 2018 Feb 24. - Publication Year :
- 2018
-
Abstract
- Agrobacterium tumefaciens can genetically transform various eukaryotic cells because of the presence of a resident tumor-inducing (Ti) plasmid. During infection, a defined region of the Ti plasmid, transfer DNA (T-DNA), is transferred from bacteria into plant cells and causes plant cells to abnormally synthesize auxin and cytokinin, which results in crown gall disease. T-DNA and several virulence (Vir) proteins are secreted through a type IV secretion system (T4SS) composed of T-pilus and a transmembrane protein complex. Three members of Arabidopsis reticulon-like B (RTNLB) proteins, RTNLB1, 2, and 4, interact with VirB2, the major component of T-pilus. Here, we have identified that other RTNLB proteins, RTNLB3 and 8, interact with VirB2 in vitro. Root-based A. tumefaciens transformation assays with Arabidopsis rtnlb3 , or rtnlb5-10 single mutants showed that the rtnlb8 mutant was resistant to A. tumefaciens infection. In addition, rtnlb3 and rtnlb8 mutants showed reduced transient transformation efficiency in seedlings. RTNLB3- or 8 overexpression transgenic plants showed increased susceptibility to A. tumefaciens and Pseudomonas syringae infection. RTNLB1-4 and 8 transcript levels differed in roots, rosette leaves, cauline leaves, inflorescence, flowers, and siliques of wild-type plants. Taken together, RTNLB3 and 8 may participate in A. tumefaciens infection but may have different roles in plants.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Arabidopsis Proteins metabolism
DNA, Bacterial genetics
Disease Susceptibility
Gene Expression
Gene Expression Regulation, Plant
Host-Pathogen Interactions
Membrane Proteins metabolism
Mutation
Organ Specificity
Plant Diseases genetics
Plant Diseases microbiology
Plants, Genetically Modified
Protein Binding
Recombinant Fusion Proteins
Yeasts genetics
Yeasts metabolism
Agrobacterium physiology
Arabidopsis genetics
Arabidopsis microbiology
Arabidopsis Proteins genetics
Membrane Proteins genetics
Transformation, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 19
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 29495267
- Full Text :
- https://doi.org/10.3390/ijms19020638