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Signaling pathways involved in zymosan phagocytosis induced by two secreted phospholipases A 2 isolated from Bothrops asper snake venom in macrophages.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2018 Jul 01; Vol. 113, pp. 575-582. Date of Electronic Publication: 2018 Mar 06. - Publication Year :
- 2018
-
Abstract
- Phagocytosis, a process involved in host defense, requires coordination of a variety of signaling reactions. MT-II, a catalytically-inactive Lys49-PLA <subscript>2</subscript> ¸ and MT-III, an active Asp49-PLA <subscript>2</subscript> isolated from Bothrops asper snake venom, activate phagocytosis in macrophages. In this study the signal pathways mediating zymosan phagocytosis, focusing in lipidic second messengers, were investigated. Macrophages collected from male Swiss mouse peritoneum were obtained 96h after i.p. injection of thioglycollate. Phagocytosis was evaluated with non-opsonized zymosan in the presence or absence of specific inhibitors. Data showed that both venom PLA <subscript>2</subscript> s increased phagocytosis. Zileuton, Etoricoxib, PACOCF <subscript>3</subscript> (5-LO, COX-2 and iPLA <subscript>2</subscript> inhibitors, respectively), as well as WEB2170 (PAF receptor antagonist) significantly reduced phagocytosis induced by both venom PLA <subscript>2</subscript> s. However, Indomethacin (COX-1/COX-2 inhibitor) and Montelukast (CysL receptor antagonist) did not affect the toxins-induced phagocytosis. Moreover, while PACOCF3 (iPLA <subscript>2</subscript> inhibitor), reduced the phagocytosis induced by MT-II and MT-III, AACOCF <subscript>3</subscript> (cPLA <subscript>2</subscript> inhibitor) significantly reduced the MT-II, but not MT-III-induced phagocytosis. These data suggest the effect of both sPLA <subscript>2</subscript> s depends on iPLA <subscript>2</subscript> and that the effect of MT-II depends on activation of cPLA <subscript>2</subscript> . COX-2 and 5-LO-derived metabolites as well as PAF are involved in the signaling events required for phagocytosis induced by both venom sPLA <subscript>2</subscript> s.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Dinoprostone biosynthesis
Intracellular Space drug effects
Intracellular Space metabolism
Macrophages metabolism
Male
Mice
Receptors, Immunologic metabolism
p38 Mitogen-Activated Protein Kinases metabolism
Bothrops
Crotalid Venoms enzymology
Macrophages cytology
Macrophages drug effects
Phospholipases A2, Secretory pharmacology
Signal Transduction drug effects
Zymosan metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 113
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 29501751
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2018.02.158