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Rapid quantification of tyrosine sulfation in therapeutic proteins.
- Source :
-
Analytical biochemistry [Anal Biochem] 2018 May 15; Vol. 549, pp. 96-98. Date of Electronic Publication: 2018 Mar 02. - Publication Year :
- 2018
-
Abstract
- Protein tyrosine sulfation (Tyr-O-SO3) is a common post-translational modification (PTM), which is important for protein function. Absolute quantitation of Tyr-O-SO3 in recombinant therapeutic proteins has been challenging. We report here an MRM method used for absolute quantitation of Tyr-O-SO3 in the hydrolysate of a recombinant Fc-fusion protein. Quantitation is achieved by monitoring the sum of two transitions: the loss of carboxylic acid from tyrosine sulfate (major transition) and sulfate group from tyrosine sulfate sodium salt. The method exhibits a good sensitivity with a limit of quantitation of 1.4 ng/mL, linearity over three orders of magnitude, good repeatability, precision and accuracy.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1096-0309
- Volume :
- 549
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 29505778
- Full Text :
- https://doi.org/10.1016/j.ab.2018.03.001