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Identification of Arcanobacterium phocae isolated from fur animals by phenotypic properties, by MALDI-TOF MS analysis and by detection of phocaelysin encoding gene phl as probable novel target.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2018 Mar; Vol. 216, pp. 45-51. Date of Electronic Publication: 2018 Feb 06. - Publication Year :
- 2018
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Abstract
- In the present study 12 Arcanobacterium phocae strains isolated from fur animals in Finland, including foxes, minks and Finnraccoons, could successfully be identified phenotypically, by matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and phocaelysin (PHL) encoding gene phl. The PHL of all 12 A. phocae strains in the present study and reference strains A. phocae DSM 10002 <superscript>T</superscript> and A. phocae DSM 10003 displayed, as typical members of the cholesterol dependent cytolysin-group of toxins, the variant undecapeptide sequence EATGLAWDPWW which appeared to be most closely related to arcanolysin of Arcanobacterium haemolyticum and pyolysin of Trueperella pyogenes. In addition, gene phl could be determined with a newly designed loop-mediated isothermal amplification (LAMP) assay. The detection of mass spectra by MALDI-TOF MS and the LAMP assay based on gene phl might help to reliably identify A. phocae in future and also elucidate the role this species plays in infections of fur animals.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Actinomycetales Infections microbiology
Animals
Arcanobacterium classification
Finland epidemiology
Foxes microbiology
Genotype
Mink microbiology
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Actinomycetales Infections veterinary
Arcanobacterium genetics
Arcanobacterium isolation & purification
Bacterial Proteins genetics
Phenotype
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2542
- Volume :
- 216
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 29519524
- Full Text :
- https://doi.org/10.1016/j.vetmic.2018.01.017