Back to Search
Start Over
Establishment of the cross-clade antigen detection system for H5 subtype influenza viruses using peptide monoclonal antibodies specific for influenza virus H5 hemagglutinin.
Establishment of the cross-clade antigen detection system for H5 subtype influenza viruses using peptide monoclonal antibodies specific for influenza virus H5 hemagglutinin.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2018 Apr 15; Vol. 498 (4), pp. 758-763. Date of Electronic Publication: 2018 Mar 15. - Publication Year :
- 2018
-
Abstract
- The H5 subtype of highly pathogenic avian influenza (H5 HPAI) viruses is a threat to both animal and human public health and has the potential to cause a serious future pandemic in humans. Thus, specific and rapid detection of H5 HPAI viruses is required for infection control in humans. To develop a simple and rapid diagnostic system to detect H5 HPAI viruses with high specificity and sensitivity, we attempted to prepare monoclonal antibodies (mAbs) that specifically recognize linear epitopes in hemagglutinin (HA) of H5 subtype viruses. Nine mAb clones were obtained from mice immunized with a synthetic partial peptide of H5 HA molecules conserved among various H5 HPAI viruses. The antigen-capture enzyme-linked immunosorbent assay using the most suitable combination of these mAbs, which bound specifically to lysed H5 HA under an optimized detergent condition, was specific for H5 viruses and could broadly detect H5 viruses in multiple different clades. Taken together, these peptide mAbs, which recognize linear epitopes in a highly conserved region of H5 HA, may be useful for specific and highly sensitive detection of H5 HPAI viruses and can help in the rapid diagnosis of human, avian, and animal H5 virus infections.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Antibodies, Monoclonal immunology
Hemagglutinin Glycoproteins, Influenza Virus immunology
Humans
Influenza A Virus, H5N1 Subtype immunology
Influenza A Virus, H5N2 Subtype immunology
Influenza A Virus, H5N8 Subtype immunology
Influenza, Human diagnosis
Influenza, Human immunology
Influenza, Human virology
Mice, Inbred BALB C
Orthomyxoviridae Infections diagnosis
Orthomyxoviridae Infections immunology
Antibodies, Monoclonal chemistry
Enzyme-Linked Immunosorbent Assay methods
Hemagglutinin Glycoproteins, Influenza Virus analysis
Influenza A Virus, H5N1 Subtype isolation & purification
Influenza A Virus, H5N2 Subtype isolation & purification
Influenza A Virus, H5N8 Subtype isolation & purification
Orthomyxoviridae Infections virology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 498
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 29524417
- Full Text :
- https://doi.org/10.1016/j.bbrc.2018.03.054