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Identification of IgG3-specific epitope that remedies problem in diagnostic IgG subclass determination due to human genetic variation.
- Source :
-
Journal of clinical pathology [J Clin Pathol] 2018 Jun; Vol. 71 (6), pp. 559-561. Date of Electronic Publication: 2018 Mar 17. - Publication Year :
- 2018
-
Abstract
- There are four subtypes of human IgG with different effector functions. Quantifying the relative amount of each IgG subtype is important for laboratory diagnosis in multiple settings. However, in an evolving landscape of the appreciation of human variability and the need for precision/personalised laboratory diagnosis, it has also been shown that there are numerous natural variants of IgG subtypes, with at least 29 having been described. It has recently been reported that commercially available polyclonal antisera to IgG3 cross react with variants of other IgG subtypes, while available monoclonal anti-IgG3 have a blind-spot for the IgG3-04 variant. Herein, we report that IgG3-04 contains an epitope in common with all known IgG3 variants and absent in other subtypes. A novel monoclonal anti-IgG3 is described that is specific to IgG3 but without any blind-spots for known IgG3 variants, providing a remedy to the problem of genetic variability of IgG3.<br />Competing Interests: Competing interests: Bloodworks NW has filed intellectual properties around the PUMA1 reagents in this report- HLH, LK, JNL and JCZ and are each employees of Bloodworks NW. XW has no competing interests to declare.<br /> (© Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.)
Details
- Language :
- English
- ISSN :
- 1472-4146
- Volume :
- 71
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of clinical pathology
- Publication Type :
- Academic Journal
- Accession number :
- 29550761
- Full Text :
- https://doi.org/10.1136/jclinpath-2018-205001