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pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor.
- Source :
-
BMC molecular biology [BMC Mol Biol] 2018 Mar 20; Vol. 19 (1), pp. 4. Date of Electronic Publication: 2018 Mar 20. - Publication Year :
- 2018
-
Abstract
- Background: Bicarbonate-based peritoneal dialysis (PD) fluids enhance the migratory capacity and damage-repair ability of human peritoneal mesothelial cells by upregulating AQP1. However, little is known about the underlying molecular mechanisms.<br />Results: Here we used HEK-293T cells to investigate the effect of pH on AQP1 gene transcription levels. We found that AQP1 mRNA levels increases with pH. Transfection of HEK-293T cells with luciferase reporter vectors containing different regions of the AQP1 promoter identified an upstream region in the AQP1 gene between - 2200 and - 2300 bp as an enhancer required for pH-mediated regulation of AQP1 expression. Site-directed mutagenesis of this specific promoter region revealed a critical region between - 2257 and - 2251 bp, and gene knock-down experiments and ChIP assays suggested that the Spi-B transcription factor SPIB is involved in pH-mediated regulation of AQP1 expression.<br />Conclusions: We identified an upstream region in the AQP1 gene and the transcription factor SPIB that are critically involved in pH-mediated regulation of AQP1 expression. These findings provide the basis for further studies on the pH- and buffer-dependent effects of PD fluids on peritoneal membrane integrity and function.
Details
- Language :
- English
- ISSN :
- 1471-2199
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 29554889
- Full Text :
- https://doi.org/10.1186/s12867-018-0104-9