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Rise and fall of outbreak-specific clone inside endemic pulsotype of Salmonella 4,[5],12:i:-; insights from high-resolution molecular surveillance in Emilia-Romagna, Italy, 2012 to 2015.
- Source :
-
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [Euro Surveill] 2018 Mar; Vol. 23 (13). - Publication Year :
- 2018
-
Abstract
- Background and aimEpidemiology of human non-typhoid salmonellosis is characterised by recurrent emergence of new clones of the pathogen over time. Some clonal lines of Salmonella have shaped epidemiology of the disease at global level, as happened for serotype Enteritidis or, more recently, for Salmonella 4,[5],12:i:-, a monophasic variant of serotype Typhimurium. The same clonal behaviour is recognisable at sub-serotype level where single outbreaks or more generalised epidemics are attributable to defined clones. The aim of this study was to understand the dynamics of a clone of Salmonella 4,[5],12:i:- over a 3-year period (2012-15) in a province of Northern Italy where the clone caused a large outbreak in 2013. Furthermore, the role of candidate outbreak sources was investigated and the accuracy of multilocus variable-number tandem repeat analysis (MLVA) was evaluated. Methods: we retrospectively investigated the outbreak through whole genome sequencing (WGS) and further monitored the outbreak clone for 2 years after its conclusion. Results: The study showed the transient nature of the clone in the population, possibly as a consequence of its occasional expansion in a food-processing facility. We demonstrated that important weaknesses characterise conventional typing methods applied to clonal pathogens such as Salmonella 4,[5],12:i:-, namely lack of accuracy for MLVA and inadequate resolution power for PFGE to be reliably used for clone tracking. Conclusions : The study provided evidence for the remarkable prevention potential of whole genome sequencing used as a routine tool in systems that integrate human, food and animal surveillance.
- Subjects :
- Bacterial Typing Techniques methods
Electrophoresis, Gel, Pulsed-Field
Humans
Italy epidemiology
Molecular Epidemiology
Molecular Typing methods
Polymorphism, Single Nucleotide
Retrospective Studies
Salmonella classification
Salmonella Infections diagnosis
Whole Genome Sequencing
DNA, Bacterial genetics
Disease Outbreaks
Endemic Diseases
Minisatellite Repeats genetics
Salmonella genetics
Salmonella isolation & purification
Salmonella Infections epidemiology
Salmonella Infections microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1560-7917
- Volume :
- 23
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 29616614
- Full Text :
- https://doi.org/10.2807/1560-7917.ES.2018.23.13.17-00375