Generation of WAe001-A-15, a human embryonic stem cell line with miR-122 doxycycline-inducible expression.

Authors :: Liu Y
Wu F
Liu D
Wei R
Abbas N
Xia J
Li D
Wang H
Zhuang Y
Guo D
Chen Y
Wu Y
Ke X
Tao J
Yang F
Lai K
Shu X
Li YX
Source :: Stem cell research [Stem Cell Res] 2018 May; Vol. 29, pp. 84-87. Date of Electronic Publication: 2018 Mar 23.
Publication Year :: 2018
Abstract: MiR-122 is the most abundant miRNA in the human liver accounting for 52% of the entire hepatic miRNome. Previous studies have demonstrated that miR-122 is a valuable therapeutic target for liver diseases, including viral hepatitis, fibrosis, steatosis, and hepatocarcinoma. Here, we constructed a miR-122 doxycycline-inducible expression human embryonic stem cell line WAe001-A-15 using the piggyBac transposon system. The cell line retained its pluripotency, in vitro differentiation potential, normal morphology, and karyotype.<br /> (Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)

Subjects :: Anti-Bacterial Agents pharmacology
Cell Line
DNA Transposable Elements
Embryonic Stem Cells drug effects
Humans
Pluripotent Stem Cells drug effects
Doxycycline pharmacology
Embryonic Stem Cells cytology
Embryonic Stem Cells metabolism
MicroRNAs biosynthesis
Pluripotent Stem Cells cytology
Pluripotent Stem Cells metabolism

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