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Genome sequence and description of Gracilibacillus timonensis sp. nov. strain Marseille-P2481 T , a moderate halophilic bacterium isolated from the human gut microflora.
- Source :
-
MicrobiologyOpen [Microbiologyopen] 2019 Feb; Vol. 8 (2), pp. e00638. Date of Electronic Publication: 2018 Apr 19. - Publication Year :
- 2019
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Abstract
- Microbial culturomics represents an ongoing revolution in the characterization of the human gut microbiota. By using three culture media containing high salt concentrations (10, 15, and 20% [w/v] NaCl), we attempted an exhaustive exploration of the halophilic microbial diversity of the human gut and isolated strain Marseille-P2481 (= CSUR P2481 =  DSM 103076), a new moderately halophilic bacterium. This bacterium is a Gram-positive, strictly aerobic, spore-forming rod that is motile by use of a flagellum and exhibits catalase, but not oxidase activity. Strain Marseille-P2481 was cultivated in media containing up to 20% (w/v) NaCl, with optimal growth being obtained at 37°C, pH 7.0-8.0, and 7.5% [w/v] NaCl). The major fatty acids were 12-methyl-tetradecanoic acid and hexadecanoic acid. Its draft genome is 4,548,390 bp long, composed of 11 scaffolds, with a G+C content of 39.8%. It contains 4,335 predicted genes (4,266 protein coding including 89 pseudogenes and 69 RNA genes). Strain Marseille-P2481 showed 96.57% 16S rRNA sequence similarity with Gracilibacillus alcaliphilus strain SG103 <superscript>T</superscript> , the phylogenetically closest species with standing in nomenclature. On the basis of its specific features, strain Marseille-P2481 <superscript>T</superscript> was classified as type strain of a new species within the genus Gracilibacillus for which the name Gracilibacillus timonensis sp. nov. is formally proposed.<br /> (© 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
- Subjects :
- Aerobiosis
Bacillaceae classification
Bacillaceae isolation & purification
Bacillaceae physiology
Base Composition
Child
Cluster Analysis
Computational Biology
Cytosol chemistry
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Fatty Acids analysis
Gastrointestinal Microbiome
Healthy Volunteers
Humans
Hydrogen-Ion Concentration
Locomotion
Male
Phylogeny
RNA, Ribosomal, 16S genetics
Senegal
Sodium Chloride metabolism
Temperature
Bacillaceae genetics
Genome, Bacterial
Sequence Analysis, DNA
Subjects
Details
- Language :
- English
- ISSN :
- 2045-8827
- Volume :
- 8
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- MicrobiologyOpen
- Publication Type :
- Academic Journal
- Accession number :
- 29675845
- Full Text :
- https://doi.org/10.1002/mbo3.638