Fucose Migration in Intact Protonated Glycan Ions: A Universal Phenomenon in Mass Spectrometry.

Authors :: Mucha E
Lettow M
Marianski M
Thomas DA
Struwe WB
Harvey DJ
Meijer G
Seeberger PH
von Helden G
Pagel K
Source :: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2018 Jun 18; Vol. 57 (25), pp. 7440-7443. Date of Electronic Publication: 2018 May 25.
Publication Year :: 2018
Abstract: Fucose is an essential deoxysugar that is found in a wide range of biologically relevant glycans and glycoconjugates. A recurring problem in mass spectrometric analyses of fucosylated glycans is the intramolecular migration of fucose units, which can lead to erroneous sequence assignments. This migration reaction is typically assigned to activation during collision-induced dissociation (CID) in tandem mass spectrometry (MS). In this work, we utilized cold-ion spectroscopy and show for the first time that fucose migration is not limited to fragments obtained in tandem MS and can also be observed in intact glycan ions. This observation suggests a possible low-energy barrier for this transfer reaction and generalizes fucose migration to an issue that may universally occur in any type of mass spectrometry experiment.<br /> (© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

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