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Hot Start Polymerase Chain Reaction (PCR).

Authors :
Green MR
Sambrook J
Source :
Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2018 May 01; Vol. 2018 (5). Date of Electronic Publication: 2018 May 01.
Publication Year :
2018

Abstract

The purpose of hot start polymerase chain reaction (PCR) is to optimize the yield of the desired amplified product in PCRs and, simultaneously, to suppress nonspecific amplification and formation of primer dimers. This is achieved by withholding an essential component of the PCR-the DNA polymerase, or the primers, for example-until the reaction mixture has been heated to a temperature that inhibits hybridization of primers to one another or to nonspecific regions of the template.<br /> (© 2018 Cold Spring Harbor Laboratory Press.)

Details

Language :
English
ISSN :
1559-6095
Volume :
2018
Issue :
5
Database :
MEDLINE
Journal :
Cold Spring Harbor protocols
Publication Type :
Academic Journal
Accession number :
29717052
Full Text :
https://doi.org/10.1101/pdb.prot095125