Back to Search
Start Over
A facile and efficient single-step approach for the fabrication of vancomycin functionalized polymer-based monolith as chiral stationary phase for nano-liquid chromatography.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2018 Jul 06; Vol. 1557, pp. 43-50. Date of Electronic Publication: 2018 May 03. - Publication Year :
- 2018
-
Abstract
- A facile single-step preparation strategy for fabricating vancomycin functionalized organic polymer-based monolith within 100μm fused-silica capillary was developed. The synthetic chiral functional monomer, i.e 2-isocyanatoethyl methacrylate (ICNEML) derivative of vancomycin, was co-polymerized with the cross-linker ethylene dimethacrylate (EDMA) in the presence of methanol and dimethyl sulfoxide as the selected porogens. The co-polymerization conditions were systematically optimized in order to obtain satisfactory column performance. Adequate permeability, stability and column morphology were observed for the optimized poly(ICNEML-vancomycin-co-EDMA) monolith. A series of chiral drugs were evaluated on the monolith in either polar organic-phase or reversed-phase modes. After the optimization of separation conditions, baseline or partial enantioseparation were obtained for series of drugs including thalidomide, colchicine, carteolol, salbutamol, clenbuterol and several other β-blockers. The proposed single-step approach not only resulted in a vancomycin functionalized organic polymer-based monolith with acceptable performance, but also significantly simplified the preparation procedure by reducing time and labor.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Carteolol analysis
Carteolol isolation & purification
Chromatography, Reverse-Phase
Colchicine analysis
Colchicine isolation & purification
Isocyanates chemistry
Methacrylates chemistry
Nanotechnology
Pharmaceutical Preparations analysis
Pharmaceutical Preparations isolation & purification
Polymerization
Reproducibility of Results
Silicon Dioxide chemistry
Stereoisomerism
Thalidomide analysis
Thalidomide isolation & purification
Chromatography, High Pressure Liquid methods
Polymers chemistry
Vancomycin chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3778
- Volume :
- 1557
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 29739612
- Full Text :
- https://doi.org/10.1016/j.chroma.2018.05.004