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Antitumor Humoral and T Cell Responses by Mucin-1 Conjugates of Bacteriophage Qβ in Wild-type Mice.
- Source :
-
ACS chemical biology [ACS Chem Biol] 2018 Jun 15; Vol. 13 (6), pp. 1668-1676. Date of Electronic Publication: 2018 May 30. - Publication Year :
- 2018
-
Abstract
- Mucin-1 (MUC1) is one of the top ranked tumor associated antigens. In order to generate effective anti-MUC1 immune responses as potential anticancer vaccines, MUC1 peptides and glycopeptides have been covalently conjugated to bacteriophage Qβ. Immunization of mice with these constructs led to highly potent antibody responses with IgG titers over one million, which are among the highest anti-MUC1 IgG titers reported to date. Furthermore, the high IgG antibody levels persisted for more than six months. The constructs also elicited MUC1 specific cytotoxic T cells, which can selectively kill MUC1 positive tumor cells. The unique abilities of Qβ-MUC1 conjugates to powerfully induce both antibody and cytotoxic T cell immunity targeting tumor cells bode well for future translation of the constructs as anticancer vaccines.
- Subjects :
- Amino Acid Sequence
Animals
Antibody Formation immunology
Bacteriophages chemistry
Cancer Vaccines chemical synthesis
Cell Line, Tumor
Humans
Immunization
Lymphoma immunology
Mice, Inbred C57BL
Microarray Analysis
Mucin-1 chemistry
T-Lymphocytes, Cytotoxic immunology
Vaccines, Synthetic chemistry
Vaccines, Synthetic immunology
Bacteriophages immunology
Cancer Vaccines immunology
Immunity, Humoral immunology
Immunoglobulin G immunology
Lymphocyte Activation immunology
Mucin-1 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1554-8937
- Volume :
- 13
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- ACS chemical biology
- Publication Type :
- Academic Journal
- Accession number :
- 29782143
- Full Text :
- https://doi.org/10.1021/acschembio.8b00313