Interaction of Yarrowia lipolytica lipase with dithiocarbamate modified magnetic carbon Fe 3 O 4 @C-NHCS 2 H core-shell nanoparticles.

Fe ₃ O ₄ @C core-shell nanoparticles were modified by (3-aminopropyl)triethoxysilane (APTES) to generate amine functionality in the surface. Then, the amine functional groups were converted to dithiocarbamate via post-modification with carbon disulfide. This nanostructure with new functional property was used to immobilize lipase (obtained from Yarrowia lipolytica U6). Biocatalytic activity of the Fe ₃ O ₄ @C-NHCS-LIP was studied in this project. The interaction of lipase and support though dithiocarbamate binder was examined in the hydrolysis of p-nitrophenyl laurate. In this paper, support showed a unique feature in the immobilization of lipase by maintaining the lipase activity, raising the stability of lipase, and reusability.
(Copyright © 2018. Published by Elsevier B.V.)