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Molecularly imprinted polymer based microtiter chemiluminescence array for determination of phenothiazines and benzodiazepines in pork.
- Source :
-
Analytical biochemistry [Anal Biochem] 2018 Aug 01; Vol. 554, pp. 9-15. Date of Electronic Publication: 2018 May 25. - Publication Year :
- 2018
-
Abstract
- In this study, a molecularly imprinted polymer based chemiluminescence array capable of simultaneous determining phenothiazines and benzodiazepines was first reported. Two polymers were coated in different wells of the conventional 96-well microtiter plate as the recognition reagents, and the added analytes competed with a horseradish peroxidase-labeled bi-hapten conjugate to bind the recognition reagents. The light signal was induced by using a highly effective luminol-H <subscript>2</subscript> O <subscript>2</subscript> -IMP system. The assay procedure consisted of only one sample-loading step prior to data acquisition. Then, the array was used to determine 4 phenothiazines and 5 benzodiazepines in pork simultaneously. The limits of detection for the 9 drugs were in a range of 0.001-0.01 ng/mL, and the recoveries from the fortified blank pork were in a range of 63.5%-94.1%. Furthermore, the array could be reused for 8 times. The detection results for some real pork samples were consistent with an ultra performance liquid chromatography method.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Chromatography, Liquid
Enzyme-Linked Immunosorbent Assay
Humans
Limit of Detection
Luminescent Measurements methods
Molecular Imprinting methods
Polymers chemistry
Sus scrofa
Benzodiazepines analysis
Drug Residues analysis
Food Contamination analysis
Phenothiazines analysis
Red Meat analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0309
- Volume :
- 554
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 29803786
- Full Text :
- https://doi.org/10.1016/j.ab.2018.05.021