[A high-precision digital images date set of larynx].

Objective: To build a digital cross-sectional images date set of human normal larynx with high-precision. Method: A fresh frozen normal human larynx was embedded using Kawamot's Film Method and then serially sectioned at 10 μm with a LeicaCM3600XP Cryomacrotome, meanwhile digital images of the block surface were recorded after every section with a Nikon D750 camera and stored respectively as JPG and RAW files. All images were cropped to display a constant area on the block surface by photoshop7 and stored as TIFF files. Result: A total of 6 656 images were recorded in 24-bit RGB resolution, the complete data file is 97.5 GB and 174 GB. After elimination of image size variation, all images were re-sampled at a final resolution of 40 μm/pixel and the complete data file is 107 GB. Conclusion: It's feasible to use the combination of Kawamot's Film Method,Digital Single Lens Reflex with high-resolution and regular block face-based alignment to build high-precision digital images date set, which could apply to some partial organs that resemble larynx.
Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.
(Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)