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Inhibition by retinoic acid of murine retrovirus-induced cellular transformation and tumor formation.
- Source :
-
Journal of the National Cancer Institute [J Natl Cancer Inst] 1985 May; Vol. 74 (5), pp. 1135-44. - Publication Year :
- 1985
-
Abstract
- The effect of all-trans-retinoic acid (RA) on cellular transformation and on tumorigenicity of retrovirally transformed cells was investigated. RA treatment of NRK and NIH/3T3 cells transformed by BALB/c murine sarcoma virus (MuSV), Kirsten murine sarcoma virus (K-MuSV), and simian sarcoma virus resulted in a significant reduction in anchorage-dependent growth of only K-MuSV-transformed NRK cells. A 62% reduction in cell number was observed at 10(-5) M RA. In contrast, anchorage-independent growth induced by each of the viruses tested was suppressed by RA. Balb/cMSV3T3 cells showed the greatest level of sensitivity with a significant reduction in anchorage-independent growth occurring at 10(-9) M RA. The level of cytoplasmic retinoic acid-binding protein (CRABP) was determined in both parent and transformed cell lines. CRABP was present at a high level in all 3T3 cell types but was absent in all NRK cell lines. For testing the antineoplastic activity of RA in vivo, Balb/cMSV3T3 cells were injected intradermally into nude mice. Subsequent treatment of the tumor sites of these animals by topical application of RA resulted in a significant reduction in both tumor incidence and tumor size, confirming the in vitro results. Analysis of the level of v-onc mRNA revealed that inhibition of retroviral transformation by RA was not due to a decrease in transcription of the v-onc genes.
- Subjects :
- Animals
Carrier Proteins analysis
Cell Division drug effects
Cell Line
Mice
Mice, Nude
Oncogenes drug effects
RNA, Messenger analysis
RNA, Viral analysis
Receptors, Retinoic Acid
Sarcoma Virus, Woolly Monkey genetics
Sarcoma Viruses, Murine genetics
Transcription, Genetic drug effects
Tretinoin therapeutic use
Cell Transformation, Viral drug effects
Skin Neoplasms prevention & control
Tretinoin pharmacology
Tumor Virus Infections prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8874
- Volume :
- 74
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of the National Cancer Institute
- Publication Type :
- Academic Journal
- Accession number :
- 2987593