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Polyethyleneimine renders mitochondrial membranes permeable by interacting with negatively charged phospholipids in them.
- Source :
-
Archives of biochemistry and biophysics [Arch Biochem Biophys] 2018 Aug 15; Vol. 652, pp. 9-17. Date of Electronic Publication: 2018 Jun 08. - Publication Year :
- 2018
-
Abstract
- Polyethyleneimines (PEIs) are used for transfection of cells with nucleic acids. Meanwhile, the interaction of PEI with mitochondria causes cytochrome c release prior to apoptosis; the mechanisms how PEI causes this permeabilization of mitochondrial membranes and the release of cytochrome c remain unclear. To clarify these mechanisms, we examined the effects of branched-type PEI and linear-type PEI, each of which was 25 kDa in size, on mitochondria. The permeabilization potency of mitochondrial membranes by branched PEI was stronger than that by linear PEI. The permeabilization by PEIs were insensitive to permeability-transition inhibitors, indicating that PEI-induced permeabilization was not attributed to permeability transition. Meanwhile, PEIs caused permeabilization of artificial lipid vesicles; again, the permeabilization potency of branched PEI was stronger than that of linear PEI. Such a difference in this potency was close to that in the case of isolated mitochondria, signifying that the PEI-induced permeabilization of mitochondrial membranes could be attributed to PEI's interaction with the phospholipid phase. Furthermore, this PEI-induced permeabilization of the lipid vesicles was observed only in the case of lipid vesicles including negatively charged phospholipids. These results indicate that PEIs interacted with negatively charged phospholipids in the mitochondrial membranes to directly lead to their permeabilization.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Calcium metabolism
Cytochromes c metabolism
Male
Mitochondria, Liver enzymology
Mitochondria, Liver metabolism
Mitochondrial Membrane Transport Proteins drug effects
Mitochondrial Membranes enzymology
Mitochondrial Membranes metabolism
Mitochondrial Permeability Transition Pore
Permeability
Rats
Rats, Wistar
Mitochondria, Liver drug effects
Mitochondrial Membranes drug effects
Phospholipids metabolism
Polyethyleneimine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0384
- Volume :
- 652
- Database :
- MEDLINE
- Journal :
- Archives of biochemistry and biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 29886045
- Full Text :
- https://doi.org/10.1016/j.abb.2018.06.003