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Bone marrow-derived mononuclear cell seeded bioresorbable vascular graft improves acute graft patency by inhibiting thrombus formation via platelet adhesion.
- Source :
-
International journal of cardiology [Int J Cardiol] 2018 Sep 01; Vol. 266, pp. 61-66. - Publication Year :
- 2018
-
Abstract
- Background: Acute thrombosis is a crucial cause of bioresorbable vascular graft (BVG) failure. Bone marrow-derived mononuclear cell (BM-MNC)-seeded BVGs demonstrated high graft patency, however, the effect of seeded BM-MNCs against thrombosis remains to be elucidated. Thus, we evaluated an antithrombotic effect of BM-MNC-seeding and utilized platelet-depletion mouse models to evaluate the contribution of platelets to acute thrombosis of BVGs.<br />Methods and Results: BVGs were composed of poly(glycolic acid) mesh sealed with poly(l-lactideco-ε-caprolactone). BM-MNC-seeded BVGs and unseeded BVGs were implanted to wild type C57BL/6 mice (n = 10/group) as inferior vena cava interposition conduits. To evaluate platelet effect on acute thrombosis, c-Mpl <superscript>-/-</superscript> mice and Pf4-Cre <superscript>+</superscript> ; iDTR mice with decreased platelet number were also implanted with unseeded BVGs (n = 10/group). BVG patency was evaluated at 2, 4, and 8 weeks by ultrasound. BM-MNC-seeded BVGs demonstrated a significantly higher patency rate than unseeded BVGs during the acute phase (2-week, 90% vs 30%, p = .020), and patency rates of these grafts were sustained until week 8. Similar to BM-MNC-seeded BVGs, C-Mpl <superscript>-/-</superscript> and Pf4-Cre <superscript>+</superscript> ; iDTR mice also showed favorable graft patency (2-week, 90% and 80%, respectively) during the acute phase. However, the patency rate of Pf4-Cre <superscript>+</superscript> ; iDTR mice decreased gradually after DTR treatment as platelet number recovered to baseline. An in vitro study revealed BM-MNC-seeding significantly inhibited platelet adhesion to BVGs compared to unseeded BVGs, (1.75 ± 0.45 vs 8.69 ± 0.68 × 10 <superscript>3</superscript> platelets/mm <superscript>2</superscript> , p < .001).<br />Conclusions: BM-MNC-seeding and the reduction in platelet number prevented BVG thrombosis and improved BVG patency, and those results might be caused by inhibiting platelet adhesion to the BVG.<br /> (Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Bone Marrow Cells physiology
Bone Marrow Transplantation trends
Cells, Cultured
Mice
Mice, Inbred C57BL
Mice, Transgenic
Polymers administration & dosage
Thrombosis diagnostic imaging
Absorbable Implants trends
Blood Vessel Prosthesis trends
Bone Marrow Transplantation methods
Platelet Adhesiveness physiology
Thrombosis prevention & control
Vascular Patency physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1874-1754
- Volume :
- 266
- Database :
- MEDLINE
- Journal :
- International journal of cardiology
- Publication Type :
- Academic Journal
- Accession number :
- 29887474
- Full Text :
- https://doi.org/10.1016/j.ijcard.2018.01.059