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NLRP3 in somatic non-immune cells of rodent and primate testes.
- Source :
-
Reproduction (Cambridge, England) [Reproduction] 2018 Sep; Vol. 156 (3), pp. 231-238. Date of Electronic Publication: 2018 Jun 15. - Publication Year :
- 2018
-
Abstract
- NLRP3 is part of the NLRP3 inflammasome and a global sensor of cellular damage. It was recently discovered in rodent Sertoli cells. We investigated NLRP3 in mouse, human and non-human primate (marmoset and rhesus macaque) testes, employing immunohistochemistry. Sertoli cells of all species expressed NLRP3, and the expression preceded puberty. In addition, peritubular cells of the adult human testes expressed NLRP3. NLRP3 and associated genes ( PYCARD , CASP1 , IL1B ) were also found in isolated human testicular peritubular cells and the mouse Sertoli cell line TM4. Male infertility due to impairments of spermatogenesis may be related to sterile inflammatory events. We observed that the expression of NLRP3 was altered in the testes of patients suffering from mixed atrophy syndrome, in which tubules with impairments of spermatogenesis showed prominent NLRP3 staining. In order to explore a possible role of NLRP3 in male infertility, associated with sterile testicular inflammation, we studied a mouse model of male infertility. These human aromatase-expressing transgenic mice ( AROM+ ) develop testicular inflammation and impaired spermatogenesis during aging, and the present data show that this is associated with strikingly elevated Nlrp3 expression in the testes compared to WT controls. Interference by aromatase inhibitor treatment significantly reduced increased Nlrp3 levels. Thus, throughout species NLRP3 is expressed by somatic cells of the testis, which are involved in testicular immune surveillance. We conclude that NLRP3 may be a novel player in testicular immune regulation.<br /> (© 2018 Society for Reproduction and Fertility.)
- Subjects :
- Adult
Animals
Aromatase genetics
Cell Line
Disease Models, Animal
Gene Expression
Humans
Immunohistochemistry
Infertility, Male etiology
Inflammasomes chemistry
Inflammation complications
Macaca mulatta
Male
Mice
Mice, Transgenic
NLR Family, Pyrin Domain-Containing 3 Protein analysis
NLR Family, Pyrin Domain-Containing 3 Protein genetics
Seminiferous Tubules chemistry
Sertoli Cells chemistry
Spermatogenesis physiology
Testis immunology
Testis metabolism
NLR Family, Pyrin Domain-Containing 3 Protein physiology
Testis cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1741-7899
- Volume :
- 156
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Reproduction (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 29907661
- Full Text :
- https://doi.org/10.1530/REP-18-0111