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Roles of Receptor-Like Cytoplasmic Kinase VII Members in Pattern-Triggered Immune Signaling.
- Source :
-
Plant physiology [Plant Physiol] 2018 Aug; Vol. 177 (4), pp. 1679-1690. Date of Electronic Publication: 2018 Jun 15. - Publication Year :
- 2018
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Abstract
- Pattern-recognition receptors (PRRs), which consist of receptor kinases (RKs) and receptor-like proteins, sense microbe- and host-derived molecular patterns associated with pathogen infection to trigger immune responses in plants. Several kinases of the 46-member Arabidopsis ( Arabidopsis thaliana ) receptor-like cytoplasmic kinase (RLCK) subfamily VII play important roles in pattern-triggered immunity, but it is unclear whether different RLCK VII members act specifically or redundantly in immune signaling. Here, we constructed nine higher order mutants of this subfamily (named rlck vii-1 to rlck vii-9 ) and systematically characterized their immune phenotypes. The mutants rlck vii-5 , - 7 , and - 8 had compromised reactive oxygen species production in response to all patterns tested, indicating that the corresponding members are broadly required for the signaling of multiple PRRs. However, rlck vii-4 was defective specifically in chitin-induced reactive oxygen species production, suggesting that RCLK VII-4 members mediate the signaling of specific PRRs. Furthermore, RLCK VII-4 members were required for the chitin-triggered activation of MAPK, demonstrating that these kinases link a PRR to MAPK activation. Moreover, we found that RLCK VII-6 and -8 also were required for RK-mediated root growth. Together, these results show that numerous RLCK VII members are involved in pattern-triggered immune signaling and uncover both common and specific roles of these kinases in plant development and immunity mediated by various RKs.<br /> (© 2018 American Society of Plant Biologists. All rights reserved.)
- Subjects :
- Arabidopsis Proteins genetics
Arabidopsis Proteins metabolism
Chitin metabolism
Disease Resistance immunology
Gene Expression Regulation, Plant immunology
Mitogen-Activated Protein Kinases immunology
Mitogen-Activated Protein Kinases metabolism
Mutation
Phylogeny
Plant Diseases immunology
Plants, Genetically Modified
Protein Kinases genetics
Protein Kinases metabolism
Pseudomonas syringae pathogenicity
Reactive Oxygen Species immunology
Reactive Oxygen Species metabolism
Receptors, Pattern Recognition genetics
Receptors, Pattern Recognition metabolism
Signal Transduction
Arabidopsis physiology
Arabidopsis Proteins immunology
Plant Immunity genetics
Protein Kinases immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 177
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 29907700
- Full Text :
- https://doi.org/10.1104/pp.18.00486