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Giardia lamblia: Identification of peroxisomal-like proteins.

Authors :
Acosta-Virgen K
Chávez-Munguía B
Talamás-Lara D
Lagunes-Guillén A
Martínez-Higuera A
Lazcano A
Martínez-Palomo A
Espinosa-Cantellano M
Source :
Experimental parasitology [Exp Parasitol] 2018 Aug; Vol. 191, pp. 36-43. Date of Electronic Publication: 2018 Jun 18.
Publication Year :
2018

Abstract

The protozoan parasite Giardia lamblia has traditionally been reported as lacking peroxisomes, organelles involved in fatty acid metabolism and detoxification of reactive oxygen species. We here report the finding with transmission electron microscopy of an oxidase activity in cytoplasmic vesicles of trophozoites and cysts of G. lamblia. These vesicles were positive to 3,3'-diaminobenzidine and to cerium chloride staining. In addition, using bioinformatic tools, two peroxisomal proteins were identified in the G. lamblia proteome: acyl-CoA synthetase long chain family member 4 (ACSL-4) and peroxin-4 (PEX-4). With confocal and immunoelectron microscopy using polyclonal antibodies both proteins were identified in cytoplasmic vesicles of trophozoites. Altogether, our results suggest for the first time the presence of peroxisomal-like proteins in the cytoplasm of G. lamblia.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1090-2449
Volume :
191
Database :
MEDLINE
Journal :
Experimental parasitology
Publication Type :
Academic Journal
Accession number :
29913139
Full Text :
https://doi.org/10.1016/j.exppara.2018.06.006