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Giardia lamblia: Identification of peroxisomal-like proteins.
- Source :
-
Experimental parasitology [Exp Parasitol] 2018 Aug; Vol. 191, pp. 36-43. Date of Electronic Publication: 2018 Jun 18. - Publication Year :
- 2018
-
Abstract
- The protozoan parasite Giardia lamblia has traditionally been reported as lacking peroxisomes, organelles involved in fatty acid metabolism and detoxification of reactive oxygen species. We here report the finding with transmission electron microscopy of an oxidase activity in cytoplasmic vesicles of trophozoites and cysts of G. lamblia. These vesicles were positive to 3,3'-diaminobenzidine and to cerium chloride staining. In addition, using bioinformatic tools, two peroxisomal proteins were identified in the G. lamblia proteome: acyl-CoA synthetase long chain family member 4 (ACSL-4) and peroxin-4 (PEX-4). With confocal and immunoelectron microscopy using polyclonal antibodies both proteins were identified in cytoplasmic vesicles of trophozoites. Altogether, our results suggest for the first time the presence of peroxisomal-like proteins in the cytoplasm of G. lamblia.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- 3,3'-Diaminobenzidine chemistry
Animals
Antibodies, Protozoan biosynthesis
Antibodies, Protozoan immunology
Blotting, Western
Cerium chemistry
Coenzyme A Ligases immunology
Coenzyme A Ligases metabolism
Computational Biology
Fluorescent Antibody Technique
Giardia lamblia enzymology
Giardia lamblia immunology
Giardia lamblia ultrastructure
Histocytochemistry
Microscopy, Confocal
Microscopy, Electron, Transmission
Microscopy, Immunoelectron
Oxidoreductases metabolism
Peroxins analysis
Peroxins immunology
Peroxisomes enzymology
Protozoan Proteins analysis
Rabbits
Staining and Labeling
Giardia lamblia chemistry
Peroxisomes chemistry
Protozoan Proteins isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2449
- Volume :
- 191
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 29913139
- Full Text :
- https://doi.org/10.1016/j.exppara.2018.06.006