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Quantitative Assessment of Nanoparticle Biodistribution by Fluorescence Imaging, Revisited.
- Source :
-
ACS nano [ACS Nano] 2018 Jul 24; Vol. 12 (7), pp. 6458-6468. Date of Electronic Publication: 2018 Jul 02. - Publication Year :
- 2018
-
Abstract
- Fluorescence-based whole-body imaging is widely used in the evaluation of nanoparticles (NPs) in small animals, often combined with quantitative analysis to indicate their spatiotemporal distribution following systemic administration. An underlying assumption is that the fluorescence label represents NPs and the intensity increases with the amount of NPs and/or the labeling dyes accumulated in the region of interest. We prepare DiR-loaded poly(lactic- co-glycolic acid) (PLGA) NPs with different surface layers (polyethylene glycol with and without folate terminus) and compare the distribution of fluorescence signals in a mouse model of folate-receptor-expressing tumors by near-infrared fluorescence whole-body imaging. Unexpectedly, we observe that fluorescence distribution patterns differ far more dramatically with DiR loading than with the surface ligand, reaching opposite conclusions with the same type of NPs (tumor-specific delivery vs predominant liver accumulation). Analysis of DiR-loaded PLGA NPs reveals that fluorescence quenching, dequenching, and signal saturation, which occur with the increasing dye content and local NP concentration, are responsible for the conflicting interpretations. This study highlights the critical need for validating fluorescence labeling of NPs in the quantitative analysis of whole-body imaging. In light of our observation, we make suggestions for future whole-body fluorescence imaging in the in vivo evaluation of NP behaviors.
- Subjects :
- Animals
Carbocyanines administration & dosage
Carbocyanines analysis
Drug Carriers analysis
Drug Carriers chemistry
Female
Fluorescent Dyes administration & dosage
Fluorescent Dyes analysis
Folic Acid chemistry
Mice
Mice, Nude
Nanoparticles analysis
Optical Imaging
Polyethylene Glycols analysis
Polyethylene Glycols chemistry
Polylactic Acid-Polyglycolic Acid Copolymer analysis
Tissue Distribution
Whole Body Imaging
Carbocyanines pharmacokinetics
Fluorescent Dyes pharmacokinetics
Nanoparticles chemistry
Neoplasms diagnostic imaging
Polylactic Acid-Polyglycolic Acid Copolymer chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1936-086X
- Volume :
- 12
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- ACS nano
- Publication Type :
- Academic Journal
- Accession number :
- 29920064
- Full Text :
- https://doi.org/10.1021/acsnano.8b02881