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Actin-binding LIM protein 1 regulates receptor activator of NF-κB ligand-mediated osteoclast differentiation and motility.
- Source :
-
BMB reports [BMB Rep] 2018 Jul; Vol. 51 (7), pp. 356-361. - Publication Year :
- 2018
-
Abstract
- Actin-binding LIM protein 1 (ABLIM1), a member of the LIM-domain protein family, mediates interactions between actin filaments and cytoplasmic targets. However, the role of ABLIM1 in osteoclast and bone metabolism has not been reported. In the present study, we investigated the role of ABLIM1 in the receptor activator of NF-κB ligand (RANKL)- mediated osteoclastogenesis. ABLIM1 expression was induced by RANKL treatment and knockdown of ABLIM1 by retrovirus infection containing Ablim1-specific short hairpin RNA (shAblim1) decreased mature osteoclast formation and bone resorption activity in a RANKL-dose dependent manner. Coincident with the downregulated expression of osteoclast differentiation marker genes, the expression levels of c-Fos and the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), critical transcription factors of osteoclastogenesis, were also decreased in shAblim1-infected osteoclasts during RANKLmediated osteoclast differentiation. In addition, the motility of preosteoclast was reduced by ABLIM1 knockdown via modulation of the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/Akt/Rac1 signaling pathway, suggesting another regulatory mechanism of ABLIM1 in osteoclast formation. These data demonstrated that ABLIM1 is a positive regulator of RANKLmediated osteoclast formation via the modulation of the differentiation and PI3K/Akt/Rac1-dependent motility. [BMB Reports 2018; 51(7): 356-361].
- Subjects :
- Animals
Bone Marrow Cells cytology
Bone Marrow Cells metabolism
Cell Movement drug effects
Cells, Cultured
LIM Domain Proteins antagonists & inhibitors
LIM Domain Proteins genetics
Mice
Mice, Inbred C57BL
Microfilament Proteins antagonists & inhibitors
Microfilament Proteins genetics
NFATC Transcription Factors genetics
NFATC Transcription Factors metabolism
Neuropeptides metabolism
Osteoclasts cytology
Osteoclasts metabolism
Phosphatidylinositol 3-Kinases metabolism
Proto-Oncogene Proteins c-akt metabolism
Proto-Oncogene Proteins c-fos genetics
Proto-Oncogene Proteins c-fos metabolism
RNA Interference
RNA, Small Interfering metabolism
Signal Transduction drug effects
rac1 GTP-Binding Protein metabolism
Cell Differentiation drug effects
LIM Domain Proteins metabolism
Microfilament Proteins metabolism
RANK Ligand pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1976-670X
- Volume :
- 51
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- BMB reports
- Publication Type :
- Academic Journal
- Accession number :
- 29921413
- Full Text :
- https://doi.org/10.5483/bmbrep.2018.51.7.106