[Effects of different intensities exercise combined with resveratrol on RBP4 in aged obese rats].

Objective: To investigate the effects of different intensity exercise combined with resveratrol on retinol binding protein 4(RBP4) mRNA and protein expression in visceral adipose tissue and plasma RBP4 concentration of aged obese rats.
Methods: Eighty male SD rats aged 3 weeks were randomly divided into the control group and the experimental group. Rats of the control group(C) were fed with ordinary feed of 6% fat ( n =12). The experimental group consisted of 68 rats, which were fed with 36%~40% high fat feed in three stages. The rat model of elderly obesity was established successfully. Twenty-four obese rats were selected and randomly divided into 4 groups, including obese group (CO), resveratrol obese group (RO), low intensity exercise combined with resveratrol group (LRO) and moderate intensity exercise combined with resveratrol group (MRO). There were 6 rats in each group. The exercise intensities of the LRO group and MRO group were (12 m/min×15 min) and (15 m/min×15 min). Every day was exercised for 60 minutes. The rats in RO, LRO or MRO were treated with resveratrol at the dose of 52.5 mg/kg·d, while the rats in the control group were fed with the same amount of purified water, continued intervention for 8 weeks. After 8 weeks, samples of blood, adipose tissue of around the kidney, testicle, vessel and visceral were collected. Blood glucose and plasma RBP4 concentration were measured. Insulin sensitivity(ISI) was calculated. The expressions of RBP4 mRNA and protein were also determined.
Results: Compared with normal (C) group, the expressions of RBP4 mRNA and protein and plasma concentration and plasma glucose of the model (CO) group were increased obviously ( P <0.05, P <0.01). ISI was decreased obviously ( P <0.05). Compared with model (CO) group, the expressions of RBP4 mRNA and protein and plasma concentration and plasma glucose of the RO and LRO and MRO groups were decreased significantly( P <0.05, P <0.01), while ISI was increased markedly ( P <0.05). Compared with the RO and LRO groups, the expressions of RBP4 mRNA and protein and plasma concentration and plasma glucose of the MRO group were decreased, while ISI was increased, but there was no significant difference.
Conclusions: Different intensities exercise combined with resveratrol could reduce the RBP4 mRNA and protein expression in visceral adipose tissue and plasma RBP4 concentrations of aged obese rats, but less affected by exercise intensity.