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Laminarin Promotes Immune Responses and Normalizes Glutamic Oxaloacetic Transaminase and Glutamic Pyruvic Transaminase Levels in Leukemic Mice In Vivo .
- Source :
-
In vivo (Athens, Greece) [In Vivo] 2018 Jul-Aug; Vol. 32 (4), pp. 783-790. - Publication Year :
- 2018
-
Abstract
- Background/aim: Laminarin, mainly found in the fronds of Laminaria, has antimicrobial characteristics and induces immune responses. However, there are no available information to show the laminarin effect on glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels in mice with leukemia in vivo.<br />Materials and Methods: Fifty normal BALB/c mice were separated randomly into five groups. Group I mice received normal diet as control. Leukemia was generated in groups II-V using WEHI-3 cells: Group II mice received normal diet as positive control; group III, IV and V mice received laminarin at 1, 2.5 and 5 mg/ml with ddH <subscript>2</subscript> O, respectively, by oral gavage every 2 days for 14 days (total of seven times). All mice were weighed during the treatment. After treatment, mice were sacrificed, blood was collected for determination of cell markers, liver and spleen samples were weighed, and spleens were used for phagocytosis and natural killer (NK) cell activity and cell proliferation using flow cytometric assay.<br />Results: Laminarin did not affect animal appearances, but increased the body weight at all doses. It reduced the weight of liver at 2.5 and 5 mg/ml and of spleen at 5 mg/ml. Laminarin increased CD3 (2.5 mg/ml) and CD19 (1 and 5 mg/ml) cell populations but reduced CD11b (5 mg/ml) cell populations, however, these did not affect Mac-3 marker level. Laminarin at 1 mg/ml increased phagocytosis by macrophages from peripheral blood mononuclear cell, but did not affect those from the peritoneal cavity. Laminarin increased NK cell cytotoxic activity at all doses and at a target ratio of 25:1 and 50:1. Laminarin did not affect B-cell proliferation, but at 5 mg/ml significantly reduced T-cell proliferation. Laminarin restored glutamate oxaloacetate transaminase (2.5 and 5 mg/ml) and glutamate pyruvate transaminase (2.5 mg/ml) levels. Based on these results, we suggest that laminarin can promote immune responses and protect against liver injury.<br /> (Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Subjects :
- Animals
Cell Proliferation drug effects
Cytotoxicity, Immunologic drug effects
Disease Models, Animal
Humans
Immunity, Cellular drug effects
Immunity, Cellular genetics
Killer Cells, Natural drug effects
Killer Cells, Natural immunology
Leukemia blood
Leukemia immunology
Leukemia pathology
Lymphocyte Activation drug effects
Macrophages drug effects
Macrophages immunology
Mice
Phagocytosis drug effects
T-Lymphocytes drug effects
Alanine Transaminase blood
Aspartate Aminotransferases blood
Glucans administration & dosage
Leukemia diet therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1791-7549
- Volume :
- 32
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- In vivo (Athens, Greece)
- Publication Type :
- Academic Journal
- Accession number :
- 29936459
- Full Text :
- https://doi.org/10.21873/invivo.11308