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Molecular Characterization of Tandem Repeat Protein 36 Gene of Ehrlichia canis Detected in Naturally Infected Dogs from Peru.
- Source :
-
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2018 Aug; Vol. 99 (2), pp. 297-302. Date of Electronic Publication: 2018 Jun 21. - Publication Year :
- 2018
-
Abstract
- Ehrlichia spp. are emerging infectious pathogens, especially in the Americas. Although Ehrlichia canis is primarily a parasite of dogs, polymerase chain reaction-confirmed human infections have been reported from Mexico, Venezuela, and Costa Rica. This study reports the presence of E. canis DNA in 13.7% of 205 dogs from urban areas in Peru and of those, five were analyzed for phylogenetic variation using the Tandem Repeat Protein 36 (TRP36) gene. The use of the TRP36 gene for such analysis was validated against 16S rRNA and heat shock protein genes using Shannon's entropy bioinformatic approach. When compared with other E. canis strains previously reported, three unique and novel E. canis strains were detected. In addition, the TRP36 amino acid tandem repeat sequences of the Peruvian strains share close similarity to an E. canis strain detected from four human blood bank samples in Costa Rica. This study reports for the first time domestic dogs infected with E. canis strains closely related to a zoonotic strain, which may be of public health concern as dogs can be chronically infected with this pathogen.
- Subjects :
- Animals
Bacterial Proteins isolation & purification
Computational Biology
DNA, Bacterial genetics
Dog Diseases epidemiology
Dog Diseases microbiology
Ehrlichia canis isolation & purification
Ehrlichiosis epidemiology
Female
Male
Peru epidemiology
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Bacterial Proteins genetics
Dogs microbiology
Ehrlichia canis genetics
Ehrlichiosis veterinary
Tandem Repeat Sequences
Subjects
Details
- Language :
- English
- ISSN :
- 1476-1645
- Volume :
- 99
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The American journal of tropical medicine and hygiene
- Publication Type :
- Academic Journal
- Accession number :
- 29943707
- Full Text :
- https://doi.org/10.4269/ajtmh.17-0776