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Fasciola hepatica induces Foxp3 T cell, proinflammatory and regulatory cytokine overexpression in liver from infected sheep during early stages of infection.
- Source :
-
Veterinary research [Vet Res] 2018 Jul 03; Vol. 49 (1), pp. 56. Date of Electronic Publication: 2018 Jul 03. - Publication Year :
- 2018
-
Abstract
- The expression of T regulatory cells (Foxp3), regulatory (interleukin [IL]-10 and transforming growth factor beta [TGF-β]) and proinflammatory (tumor necrosis factor alpha [TNF-α] and interleukin [IL]-1β) cytokines was quantified using real time polymerase chain reaction (qRT-PCR) in the liver of sheep during early stages of infection with Fasciola hepatica (1, 3, 9, and 18 days post-infection [dpi]). Portal fibrosis was also evaluated by Masson's trichrome stain as well as the number of Foxp3 <superscript>+</superscript> cells by immunohistochemistry. Animals were divided into three groups: (a) group 1 was immunized with recombinant cathepsin L1 from F. hepatica (FhCL1) in Montanide adjuvant and infected; (b) group 2 was uniquely infected with F. hepatica; and (c) group 3 was the control group, unimmunized and uninfected. An overexpression of regulatory cytokines of groups 1 and 2 was found in all time points tested in comparison with group 3, particularly at 18 dpi. A significant increase of the number of Foxp3 <superscript>+</superscript> lymphocytes in groups 1 and 2 was found at 9 and 18 dpi relative to group 3. A progressive increase in portal fibrosis was found in groups 1 and 2 in comparison with group 3. In this regard, group 1 showed smaller areas of fibrosis than group 2. There was a significant positive correlation between Foxp3 and IL-10 expression (by immunohistochemistry and qRT-PCR) just as between portal fibrosis and TGF-β gene expression. The expression of proinflammatory cytokines increased gradually during the experience. These findings suggest the induction of a regulatory phenotype by the parasite that would allow its survival at early stages of the disease when it is more vulnerable.
- Subjects :
- Animals
Cytokines metabolism
Fascioliasis immunology
Fascioliasis virology
Forkhead Transcription Factors metabolism
Liver parasitology
Sheep
Sheep Diseases virology
Cytokines genetics
Fasciola hepatica physiology
Fascioliasis veterinary
Forkhead Transcription Factors genetics
Gene Expression
Sheep Diseases immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1297-9716
- Volume :
- 49
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 29970179
- Full Text :
- https://doi.org/10.1186/s13567-018-0550-x