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Salidroside ameliorates autophagy and activation of hepatic stellate cells in mice via NF-κB and TGF-β1/Smad3 pathways.
- Source :
-
Drug design, development and therapy [Drug Des Devel Ther] 2018 Jun 22; Vol. 12, pp. 1837-1853. Date of Electronic Publication: 2018 Jun 22 (Print Publication: 2018). - Publication Year :
- 2018
-
Abstract
- Purpose: Liver fibrosis is commonly seen and a necessary stage in chronic liver disease. The aim of this study was to explore the effect of salidroside on liver fibrosis in mice and its potential mechanisms.<br />Materials and Methods: Two mouse liver fibrosis models were established by intraperitoneal injection of carbon tetrachloride (CCl <subscript>4</subscript> ) for 8 weeks and bile duct ligation for 14 days. Salidroside was injected intraperitoneally at doses of 10 and 20 mg/kg once a day. Gene and protein expression levels were determined by quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, Western blot, immunohistochemistry, and immunofluorescence.<br />Results: Salidroside inhibited the production of extracellular matrix (ECM) and regulated the balance between MMP2 and TIMP1 and, therefore, alleviated liver fibrosis in the two fibrosis models. Salidroside reduced the production of transforming growth factor (TGF)-β1 in Kupffer cells and hepatic stellate cells (HSCs) via the nuclear factor-κB signaling pathway and, therefore, inhibited the activation of HSCs and autophagy by downregulation of the TGF-β1/Smad3 signaling pathway.<br />Conclusion: Salidroside can effectively attenuate liver fibrosis by inhibiting the activation of HSCs in mice.<br />Competing Interests: Disclosure The authors report no conflicts of interest in this work.
- Subjects :
- Animals
Liver Cirrhosis drug therapy
Male
Mice
Mice, Inbred C57BL
Autophagy drug effects
Glucosides pharmacology
Hepatic Stellate Cells drug effects
NF-kappa B physiology
Phenols pharmacology
Signal Transduction drug effects
Smad3 Protein physiology
Transforming Growth Factor beta1 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1177-8881
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- Drug design, development and therapy
- Publication Type :
- Academic Journal
- Accession number :
- 29970958
- Full Text :
- https://doi.org/10.2147/DDDT.S162950