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Dual-Modal Split-Type Immunosensor for Sensitive Detection of Microcystin-LR: Enzyme-Induced Photoelectrochemistry and Colorimetry.
- Source :
-
Analytical chemistry [Anal Chem] 2018 Aug 07; Vol. 90 (15), pp. 9606-9613. Date of Electronic Publication: 2018 Jul 20. - Publication Year :
- 2018
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Abstract
- Microcystins, the lethal cyanotoxins from Microcystis aeruginosa, can inhibit the activity of protein phosphatase and promote liver tumors. Herein, a dual-modal split-type immunosensor was constructed to detect microcystin-LR (MC-LR), based on the photocurrent change of CdS/ZnO hollow nanorod arrays (HNRs) and the blue shift of the surface plasmon resonance peak from Au nanobipyramids@Ag. By using mesoporous silica nanospheres as the carrier to immobilize secondary antibody and DNA primer, a hybridization chain reaction was adopted to capture alkaline phosphatase, while its catalytic reaction product, ascorbic acid, exhibited dual functions. The detailed mechanism was investigated, showing that ascorbic acid can not only act as the electron donor to capture the holes in CdS/ZnO-HNRs, leading to the increase photocurrent, but also as the reductant to form silver shells on Au nanobipyramids, generating multiply vivid color variations and blue shifts. Compared with the traditional photoelectrochemical immunosensor or colorimetric method for MC-LR, a more accurate and reliable result can be obtained, due to different mechanisms and independent signal transduction. Therefore, this work can not only propose a new dual-modal immunosensor for MC-LR detection but also provide innovative inspiration for constructing sensitive, accurate, and visual analysis for toxins.
- Subjects :
- DNA Primers chemistry
Gold chemistry
Immunoassay methods
Limit of Detection
Marine Toxins
Metal Nanoparticles chemistry
Microcystis chemistry
Nanotubes ultrastructure
Silver chemistry
Antibodies, Immobilized chemistry
Cadmium Compounds chemistry
Colorimetry methods
Microcystins analysis
Nanotubes chemistry
Selenium Compounds chemistry
Surface Plasmon Resonance methods
Zinc Oxide chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 90
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 29985599
- Full Text :
- https://doi.org/10.1021/acs.analchem.8b02546