Back to Search
Start Over
An ETHYLENE RESPONSE FACTOR-MYB Transcription Complex Regulates Furaneol Biosynthesis by Activating QUINONE OXIDOREDUCTASE Expression in Strawberry.
- Source :
-
Plant physiology [Plant Physiol] 2018 Sep; Vol. 178 (1), pp. 189-201. Date of Electronic Publication: 2018 Jul 09. - Publication Year :
- 2018
-
Abstract
- 4-Hydroxy-2,5-dimethyl-3(2H)-furanone is a major contributor to the aroma of strawberry ( Fragaria × ananassa ) fruit, and the last step in its biosynthesis is catalyzed by strawberry quinone oxidoreductase (FaQR). Here, an ethylene response factor (FaERF#9) was characterized as a positive regulator of the FaQR promoter. Linear regression analysis indicated that FaERF#9 transcript levels were correlated significantly with both FaQR transcripts and furanone content in different strawberry cultivars. Transient overexpression of FaERF#9 in strawberry fruit significantly increased FaQR expression and furaneol production. Yeast one-hybrid assays, however, indicated that FaERF#9 by itself did not bind to the FaQR promoter. An MYB transcription factor (FaMYB98) identified in yeast one-hybrid screening of the strawberry cDNA library was capable of both binding to the promoter and activating the transcription of FaQR by ∼5.6-fold. Yeast two-hybrid assay and bimolecular fluorescence complementation confirmed a direct protein-protein interaction between FaERF#9 and FaMYB98, and in combination, they activated the FaQR promoter 14-fold in transactivation assays. These results indicate that an ERF-MYB complex containing FaERF#9 and FaMYB98 activates the FaQR promoter and up-regulates 4-hydroxy-2,5-dimethyl-3(2H)-furanone biosynthesis in strawberry.<br /> (© 2018 American Society of Plant Biologists. All rights reserved.)
- Subjects :
- Fragaria genetics
Fruit genetics
Fruit metabolism
Gene Expression Profiling
Gene Expression Regulation, Plant
Multiprotein Complexes metabolism
NAD(P)H Dehydrogenase (Quinone) genetics
Plant Proteins genetics
Promoter Regions, Genetic genetics
Protein Binding
Transcription Factors genetics
Two-Hybrid System Techniques
Fragaria metabolism
Furans metabolism
NAD(P)H Dehydrogenase (Quinone) metabolism
Plant Proteins metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 178
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 29987002
- Full Text :
- https://doi.org/10.1104/pp.18.00598