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Intravital microscopy of collective invasion plasticity in breast cancer.
- Source :
-
Disease models & mechanisms [Dis Model Mech] 2018 Aug 23; Vol. 11 (9). Date of Electronic Publication: 2018 Aug 23. - Publication Year :
- 2018
-
Abstract
- Cancer invasion programs are adaptive by switching between metastatic collective and single-cell dissemination; however, current intravital microscopy models for epithelial cancer in mice fail to reliably recreate such invasion plasticity. Using microimplantation of breast cancer spheroids into the murine mammary fat pad and live-cell monitoring, we show microenvironmental conditions and cytoskeletal adaptation during collective to single-cell transition in vivo E-cadherin-expressing 4T1 and E-cadherin-negative MMT tumors both initiated collective invasion along stromal structures, reflecting invasion patterns in 3D organotypic culture and human primary ductal and lobular carcinoma. Collectively invading cells developed weakly oscillatory actin dynamics, yet provided zones for single-cell transitions with accentuated, more chaotic actin fluctuations. This identifies collective invasion in vivo as a dynamic niche and efficient source for single-cell release.<br />Competing Interests: Competing interestsThe authors declare no competing or financial interests.<br /> (© 2018. Published by The Company of Biologists Ltd.)
- Subjects :
- Actins metabolism
Animals
Cell Line, Tumor
Cell Proliferation
Female
HEK293 Cells
Humans
Imaging, Three-Dimensional
Mammary Neoplasms, Animal blood supply
Mice, Inbred BALB C
Neoplasm Invasiveness
Neoplasm Metastasis
Neovascularization, Pathologic pathology
Stromal Cells pathology
Cell Plasticity
Intravital Microscopy
Mammary Neoplasms, Animal diagnostic imaging
Mammary Neoplasms, Animal pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1754-8411
- Volume :
- 11
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Disease models & mechanisms
- Publication Type :
- Academic Journal
- Accession number :
- 29997220
- Full Text :
- https://doi.org/10.1242/dmm.034330