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Influenza A Virus Negative Strand RNA Is Translated for CD8 + T Cell Immunosurveillance.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2018 Aug 15; Vol. 201 (4), pp. 1222-1228. Date of Electronic Publication: 2018 Jul 16. - Publication Year :
- 2018
-
Abstract
- Probing the limits of CD8 <superscript>+</superscript> T cell immunosurveillance, we inserted the SIINFEKL peptide into influenza A virus (IAV)-negative strand gene segments. Although IAV genomic RNA is considered noncoding, there is a conserved, relatively long open reading frame present in segment 8, encoding a potential protein termed NEG8. The biosynthesis of NEG8 from IAV has yet to be demonstrated. Although we failed to detect NEG8 protein expression in IAV-infected mouse cells, cell surface K <superscript>b</superscript> -SIINFEKL complexes are generated when SIINFEKL is genetically appended to the predicted C terminus of NEG8, as shown by activation of OT-I T cells in vitro and in vivo. Moreover, recombinant IAV encoding of SIINFEKL embedded in the negative strand of the neuraminidase-stalk coding sequence also activates OT-I T cells in mice. Together, our findings demonstrate both the translation of sequences on the negative strand of a single-stranded RNA virus and its relevance in antiviral immunosurveillance.
- Subjects :
- Animals
HeLa Cells
Humans
Mice
Mice, Inbred C57BL
Mice, Transgenic
Orthomyxoviridae Infections immunology
Protein Biosynthesis physiology
RNA, Viral genetics
CD8-Positive T-Lymphocytes immunology
Immunologic Surveillance immunology
Influenza A virus genetics
Influenza A virus immunology
RNA, Viral immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 201
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 30012850
- Full Text :
- https://doi.org/10.4049/jimmunol.1800586