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A chemiluminescence-based catalase assay using H 2 O 2 -sensitive CdTe quantum dots.
- Source :
-
Mikrochimica acta [Mikrochim Acta] 2018 Jul 16; Vol. 185 (8), pp. 376. Date of Electronic Publication: 2018 Jul 16. - Publication Year :
- 2018
-
Abstract
- A method is described for the chemiluminescence based determination of the activity of catalase (CAT) using H <subscript>2</subscript> O <subscript>2</subscript> -sensitive CdTe quantum dots (QDs). It is based on the finding that the chemiluminescence (CL) of the CdTe/H <subscript>2</subscript> O <subscript>2</subscript> system is reduced due to the consumption of H <subscript>2</subscript> O <subscript>2</subscript> by the catalytic action of CAT. The Michaelis constant is calculated to be 519 ± 27 mM, showing the potential of the method to accurately measure the K <subscript>m</subscript> compared to the standard method. The method does not require QDs to be conjugated to biological/organic molecules and therefore is considered to be a rapid and convenient method for determination of CAT in real samples. At an incubation time of 2 s, the LOD was calculated to be 4.5 unit/mL, with a linear range from 6 to 400 unit/mL. The assay is sensitive, simple, and suitable for practical applications. Graphical abstract Schematic representation of chemiluminescence-based catalase U(CAT) assay using the CdSe QD/H <subscript>2</subscript> O <subscript>2</subscript> system. The reduction of H <subscript>2</subscript> O <subscript>2</subscript> is reflected by the chemiluminescence of the QDs. A mechanism is put forward based on the changes in chemiluminescence intensity of the QDs by the consumption of H <subscript>2</subscript> O <subscript>2</subscript> due to the catalytic action of CAT.
Details
- Language :
- English
- ISSN :
- 1436-5073
- Volume :
- 185
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Mikrochimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 30014207
- Full Text :
- https://doi.org/10.1007/s00604-018-2912-5